A Tomography-Like Real-Time Imaging of Neuronal Activity in the Intact Brain.
Abstract
Use of voltage-sensitive dyes and of optical recording of neuronal activity is a potentially powerful technique for investigating the development, organization, function, and recovery from malfunction of the mammalian cortex in vivo. The major advantage of optical recording is the feasibility to record single cells or population activity, from thousands of loci, simultaneously. We also used the optical methodology to address biological questions related to the normal and pathological function of CNS myelinated axons. These findings are discussed in detail. We also developed applications of voltage sensitive dyes in two new directions: New voltage sensitive dyes were designed and synthesized for iontophoretic injection into single cells. With this approach we have demonstrated, for the first time, the feasibility of recording post synaptic potential from dendritic processes in situ. The same probes were also used for a second application; extracellular injection of these dye in conjunction with reterograde and/or anterograde labeling of specfic pathways in the brain, have been demonstrated. These results are also discussed. Keywords: Optical image, Neuronal networks, Neuronal assemblies; Visual cortex.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 30, 1986
- Accession Number
- ADA177377
Entities
People
- Amiram Grinvald