Exploration of Binding and Toxic Site of Botulinum Neurotoxin

Abstract

Roles of the heavy and light chains of the neurotoxin (NT) in neuroparalysis were examined in two systems. (1) The heavy chin first binds to the specific sites (receptors) on the nerve terminals. This binding is necessary for the light chain to be fixed at these specific sites. Then the light chain (or combination of the light and heavy chain) induces paralysis through a mechanism very similar to that of the parent dichain nt. (2) The isolated heavy chain forms channels in planar bilayer membranes. These channels have pH and voltage dependent gating properties. Planter - lunbrical of the hind paw of the mouse were introduced as neuromuscular preparation for studying botulinum nt induced paralysis.

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Document Details

Document Type
Technical Report
Publication Date
Jan 30, 1986
Accession Number
ADA177585

Entities

People

  • B. R. Dasgupta

Organizations

  • University of Wisconsin–Madison

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biomedical Research
  • Brain
  • Buildings And Structures
  • Contracts
  • Diseases And Disorders
  • Membranes
  • Nerves
  • Neurosciences
  • Neurotoxins
  • Paralysis
  • Peripheral Nervous System
  • Phrenic Nerves
  • Synaptosomes
  • Technical Information Centers
  • Terminals
  • Wisconsin

Readers

  • Molecular and Cellular Biochemistry