Studies on the Immunochemical Techniques for Detection of Selected Fungal and Dinoflagellate Toxins

Abstract

Methods for the production of antibodies in rabbits against diacetoxysirpenol (DAS), deoxyverrucarol (DOVE), acetyl-deoxynivalenol (Ac-DON) and T-2 toxin metabolities including were developed. A number of tritiated trichothecene mycotoxins of high specific radioactivity were prepared. A new improved method for the production of antibodies against T-2 toxin and DAS in rabbits was developed. With the availability of different antibodies, sensitive and specific RIAs for these mycotoxins were developed. An indirect ELISA for analysis of saxitoxin (STX) was developed. The ability produce T-2 toxin and DAS by 18 fusaria was studied by RIA. Limited efforts for production of monoclonal antibodies for STX and T-2 toxin were made; but no stable clones were obtained. 8) The metabolism of T-2 toxin in vitro was found to be affected by esterase inhibitors greatly. Routine production of antibodies against T-2 toxin and DAS continued with the new T-2 toxin and DAS derivatives. Some immunochemical reagents were made and delivered to the USAMRIID.

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Document Details

Document Type
Technical Report
Publication Date
Aug 31, 1985
Accession Number
ADA177666

Entities

People

  • F. S. Chu

Organizations

  • University of Wisconsin–Madison

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Albumins
  • Antibodies
  • Availability
  • Biomedical Research
  • Blood
  • Buildings And Structures
  • Detection
  • Fungi
  • Immune Serums
  • Incubation
  • Inhibitors
  • Laboratory Animals
  • Metabolism
  • Metabolites
  • Production
  • Proteins

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular and Cellular Biochemistry
  • Mycotoxin ecology in Amazonian ecosystems.