Studies on the Immunochemical Techniques for Detection of Selected Fungal and Dinoflagellate Toxins.
Abstract
Conditions for improving antibody production against T-2 toxin were studied. Methods for the production of antibodies in rabbits against diacetoxyscirpenol (DAS), deoxyverrucarol (DOVE), acetyl-deoxynivalenol (Ac-DON) and T-2 toxin metabolities were developed. A new improved method for the production of antibodies against T-2 toxin and DAS in rabbits was developed. A generic-type antibody which cross-reacted with most type A trichothecene mycotoxins was obtained. Saxitoxin antibody was raised after immunizing rabbits with STX-HCHO-BSA. A number of tritiated trichothecene mycotoxins of high specific radioactivity were prepared. With the availability of different antibodies, sensitive and specific RIAS for these mycotoxins (DAS, DOVE, and DON) were developed. A direct competitive ELISA for DAS was developed. An indirect ELISA which permits to detect T-2 toxin in urine, serum and milk was established. An indirect ELISA for simultaneous analysis of HT-2 and T-2 toxin in urine was also established. An indirect ELISA for analysis of saxitoxin (STX) was developed. Antibodies against T-2 toxin and DAS have been used to localize T-2 toxin in different tissues and organs of mice by a immunohistochemical technique. The ability of producing T-2 toxin and DAS by 18 Fusaria was studied. Limited efforts for production of monoclonal antibodies for STX and T-2 toxin were made. Metabolism of T-2 toxin in vitro was found to be affected by esterase inhibitors greatly. Routine production production of antibodies against T-2 toxin and DAS continued. The immunochemical reagents prepared for a delivered to the USAMRIID are summarized.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 15, 1985
- Accession Number
- ADA177667
Entities
People
- F. S. Chu
Organizations
- University of Wisconsin–Madison