Exploration of Binding and Toxic Site of Botulinum Neurotoxin

Abstract

The research was directed to identify and characterize the three active regions of a large protein, botulinum neurotoxin, responsible for 1) binding to the receptors, 2) forming channels on membrane, and 3) blocking acetylcholine release. The heavy chain of the neurotoxin first binds to the specific sites (receptors) on the nerve terminals. This binding is necessary for the light chain to be fixed at these specific sites. Then the light chain induces paralysis. The heavy chain, but not the light chain, forms channels in planar bilayer membranes. These channels have pH and voltage dependent gating properties. Planter nerves-lumbrical of the hind paw of the mouse were introduced as neuromuscular preparation for studying the neurotoxin induced paralysis, particularly for electron microscopy of the binding of radiolabelled neurotoxin. Keywords: neuromuscular preparation; antagonism of neurotoxin; acetylcholine release blockage.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jan 30, 1986
Accession Number
ADA177694

Entities

People

  • B. R. Dasgupta

Organizations

  • University of Wisconsin–Madison

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biomedical Research
  • Contracts
  • Covalent Bonds
  • Diseases And Disorders
  • Electron Microscopy
  • Electrons
  • Infectious Diseases
  • Laboratory Animals
  • Membrane Lipids
  • Membranes
  • Nerves
  • Neurotoxins
  • Paralysis
  • Peripheral Nervous System
  • Phrenic Nerves
  • Technical Information Centers

Fields of Study

  • Biology

Readers

  • Cardiovascular Physiology
  • Immunology
  • Neurodegenerative Parkinson's Disease and Rickettsial Disease handbook, including the data level of dopamine, BC, neurons, and PD.

Technology Areas

  • Microelectronics