Rapid Detection of Enveloped Viruses.
Abstract
This report discusses the continuing effort to enhance the sensitivity of type A influenza virus detection systems utilizing the monoclonal antibodies to M-protein. Combinations of purified monoclonal antibodies to M-protein used as capture antibodies for type A influenza viruses were superior to the use of a single monoclonal antibody for virus capture. The results correlate with our findings on epitope analysis of the monoclonal antibodies to M-protein. Therefore, it would be predicted that combinations of monoclonal antibodies recognizing different eptitopes would provide maximal sensitivity. A Western blot techniuqe utilizing enzyme immunoassay visualization was developed. All monoclonal antibodies previously found to react with M-protein by microtiter ELISA assay also reacted with M-protein by the Western blot techique. Collaborative work on the production of immunoreactive peptide segments of M-protein is important from at least two aspects. Synthetic peptide segments of M-protein can be used in ELISA assays as adsorbents to determine the precise epitopes seen by monoclonal antibodies to M-protein and synthetic peptides of regions of M-protein may serve as adsorbent antigen in place of M-protein in viral detection systems based on competitive inhibition assay. Keywords: ELISA (Enzyme Linked Immunosorbent Assay)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 26, 1986
- Accession Number
- ADA177818
Entities
People
- Doris J. Bucher
Organizations
- Mount Sinai Hospital