Exploration of Binding and Toxic Site of Botulinum Neurotoxin
Abstract
The dissection of the hind paws of a mouse provide nerve muscle preparation (Planter nerves-lumbrical muscle preparation). These are thin, short and flat. The location of neuromuscular junctions is highly predictable. These preparations were introduced for the first time for studying botulinum neurotoxin induced paralysis; primarily for electron microscopy of the binding of radiolabelled neurotoxin. The heavy chain (Mr 97,000) isolated from type A neurotoxin (Mr 145,000) significantly antagonized the poisoning effect of the type A as well as type E neurotoxin. The heavy chain thus appeared to bind to the receptors on neuromuscular junction to which the whole neurotoxin binds. This was the first demonstration of such competition using neuromuscular junction preparation and pharmacological activity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 30, 1986
- Accession Number
- ADA177883
Entities
People
- B. R. Dasgupta
Organizations
- University of Wisconsin–Madison