Molecular and Biological Properties of an Immunopotentiating Complex Polysaccharide Adjuvant Produced by a Gliding Bacterium.
Abstract
A species of the gliding bacteria Cytophaga produces an exopolymer, designated GBA, that potentiates immune reactions. We showed previously that GBA augments primary immune responses in vitro, causes B lymphocytes to proliferate and secrete immunoglobulin, and induces macrophages to secrete Interleukin 1. GBA is prepared from culture supernatants of Cytophaga. Chemical analysis of these molecules indicates that they are large particulate polymers of amino sugars. In the period covered by this report we examined GBA for additional immunomodulatory activities. We found that GBA stimulates cultured human peripheral blood cells to secrete tumor necrotizing factor alpha and that GBA augments endogenous colony forming the pre-B cell line WEHI 231. We also found that GBA augments endogenous colony forming units in radiation-treatment mice and that it protects mice from otherwise lethal infectious doses of selected strains of E. coli. Other experiments showed that GBA is an immunological adjuvant in vivo. Primary humoral responses of mice immunized with protein antigens in combination with GBA were significantly higher than those of mice immunized with antigen alone. Moreover preliminary experiments showed that GBA augments antigen-specific immunological memory. Keywords: Cytophaga; Immunoenhancing; Radiation; Protection; Infection; ELISA (Enzyme Linked Immunosorbent Assay).
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 27, 1987
- Accession Number
- ADA177915
Entities
People
- Robert I. Mishell
- William R. Usinger
Organizations
- University of California, Berkeley