Computer-Aided Design of Thermostable Proteins.

Abstract

Strategies are being developed for computer-aided protein design. The strategies emphasize simple geometric aspects of protein structure, and a computer program (PROTEUS) allows us to systematically test a very large number of alternative sequences and conformations. In preliminary work we had added a disulfide band to the N-terminal domain of lambda repressor. This increases the thermal stability by 8 C. Combining this disulfide bond with two glycine to alanine changes in alpha helix 3 variant that is 16 C more stable than the wild type proteins. Attempts are in progress to improve electrostatic interactions and the stacking of aromatic residues. Keywords: Directed mutagenesis/protein engineering.

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Document Details

Document Type
Technical Report
Publication Date
Mar 13, 1987
Accession Number
ADA178181

Entities

People

  • Carl O. Pabo

Organizations

  • Johns Hopkins University

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Artificial Intelligence
  • Biochemistry
  • Biology
  • Computer Programming
  • Computer Programs
  • Computer-Aided Design
  • Computers
  • Coordinate Systems
  • Databases
  • Engineering
  • Expert Systems
  • Genetics
  • Protein Engineering
  • Sequences
  • Terminals
  • Thermal Stability

Readers

  • Molecular and Cellular Biochemistry
  • Software Engineering
  • Systems Analysis and Design

Technology Areas

  • Biotechnology