Development of General Antisera for Trichothecanes

Abstract

Antibodies with narrow structural requirements of binding are useful in specific assays. Antibodies which bind a variety of structurally related compounds could be used in detection of classes of compounds. This project is exploring the relationship between immunogen (hapten) structure and the selectivity of resulting antibodies to various of the trichothencenes. Immunogens are synthesized and used to immunize rabbits. The polyclonal sera are then examined for their selectivity for various trichothecene compounds before attempts are made to prepare monoclonal antibodies. The first position examined for linkage to protein was the 8-position of the trichothecene molecule. Two simple syntheses of T-2 toxin from readily available anguidine were developed. The synthesis of the 4-keto analog of HT-2 toxin was achieved. Keywords: Mycotoxins; Trichothecanes; Synthesis; Radioisotope; Immunoassay; EIA(Enzyme Immunoassay), RIA(Radioimmunoassay).

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1986
Accession Number
ADA178266

Entities

People

  • C. C. Whisnant
  • C. E. Cook
  • M. C. Wani

Organizations

  • RTI International

Tags

DTIC Thesaurus Topics

  • Acetic Acid
  • Albumins
  • Alcohols
  • Aluminum
  • Aluminum Hydride
  • Anhydrides
  • Biomedical Research
  • Chemical Synthesis
  • Chemistry
  • Chlorides
  • Detection
  • Fungi
  • Immunoassay
  • Organic Chemistry
  • Physical Properties
  • Plastic Explosives
  • Spectra

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and Cellular Biochemistry