Chemiluminescence from AqueousMicellar Systems.
Abstract
A. Chemical and Enzymatic Triggering of 1,2-Dioxetanes A series of dioxetanes which are thermally stable at ambient temperature but which can be triggered for efficient chemiluminescence on demand by the addition of the appropriate chemical catalyst or enzyme have been discovered the process involves the removal of a protecting group from a stable form of the dioxetane to generate an unstable aryloxide intermediate which decomposes spontaneously to yield the luminescence. B. Chemiluminescence from Micellar System cationic surfactants such as cetytrimethylammonium bromide (CATB) can be used to significantly enhance rates of chemically triggered luminescence from appropriately substituteddioxetanes in aqueous solution. For example, CATB catalyzes the base-induced cleavage of an acetate-substituted dioxetane. Fluoride-triggering of silyloxy dioxetanes is also accelerated in the micelle. The electrostatic attraction of the cationic head group and the anionic reagent provides the observed micellar catalysis. C. Effects of Heteroatom substitutents on the properties of dioxetanes resulted in the first report of activation parameters and rates of decomposition for unstable nitrogen-and sulfur-substituted dioxetanes. The results provide support for a mechanism for decomposition involving intramolecular electron transfer. D. Hematoporophyrin-Chemiluminescence Cancer Therapy-Photoradiation therapy using a hematoporphyrin derivative (HPD) and visible light has shown considerable promise as an effective treatment for a wide variety of cancers. This procedure is being used clinically in many hospitals on an experimental basis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 24, 1987
- Accession Number
- ADA178503
Entities
People
- A. P. Schaap
Organizations
- Wayne State University