Human Hybriodomas for Exotic Antigens.
Abstract
This is the first annual report under a 3-year contract to develop methods for generating human hybridomas in vitro that can produce antibodies specific for exotic antigens which could not be produced by normal in vivo immunization procedures. The basic methodology for generating human hybridoma cell lines was available. However, several minor modifications were required in order to adapt methods for use with peripheral blood lymphocytes (PBL) obtained by lymphapharesis of normal human donors. Much of this first years work has been directed at establishing optimal conditions for recovering hybridomas from PBL. The methods now in hand allow the recovery of 5 stable hybridomas per 1,009000 PBL; given that 10% of PBL are B-cells and roughly 10% of B-cells can be captured as hybridomas, this means that for every 2,000 potential B-cell targets that are recoverable in principle, one is obtained. Considering hte 1,000-fold excess of irrelevant cells present during the fusion process, a recovery of 1 hybrid for every 2,000 potential targets makes the next step of the project fully feasible. Keywords: Monoclomal antibodies; antigens.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1986
- Accession Number
- ADA178755
Entities
People
- Melvin Cohn
Organizations
- Salk Institute for Biological Studies