Carboxyalkylated Hemoglobin as a Potential Blood Substitute.

Abstract

Carboxymethylated hemoglobin A has been prepared for possible use as a potential blood substitute. The covalently attached carboxymethyl groups on the terminal amino groups of hemoglobin lead to a significantly lowered oxygen affinity. The allosteric effector carbon dioxide is also known to lead to a lower oxygen affinity. The stable carboxymethyl derivative is considered to be an analog of the adduct of carbon dioxide and hemoglobin. The conclusion is supported by X-ray diffraction analysis. Studies on the cross-linking of the derivative by glycolaldehyde have been initiated. In preliminary studies, the cross-linked carboxymethyl hemoglobin was found to have an oxygen affinity lower than that of unmodified hemoglobin but higher than that of the carboxymethylated derivative. Further studies on the characterization of the cross-linked species are in progress. Keywords: Blood Surrogate, Carboxyalkylation.

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Document Details

Document Type
Technical Report
Publication Date
Jan 24, 1987
Accession Number
ADA178946

Entities

People

  • James M. Manning

Organizations

  • The Rockefeller University

Tags

DTIC Thesaurus Topics

  • Acetic Acid
  • Amino Acids
  • Analogs
  • Blood Substitutes
  • Carbon Dioxide
  • Chemistry
  • Chlorides
  • Classification
  • Diffraction
  • Electrophoresis
  • Filtration
  • Gel Electrophoresis
  • Hemoglobin
  • Molecules
  • Proteins
  • X Rays
  • X-Ray Diffraction

Fields of Study

  • Chemistry

Readers

  • Electrochemical Engineering/ Fuel Cell Technologies
  • Molecular and Cellular Biochemistry