Biomedical Applications of the Free Electron Laser.
Abstract
This study involves seven separate projects. The areas approached are the: modeling of porphyrin uptake, sequestration and photoxicity genetic in identical genetic cell lines, the photosensitizing properties of merocyanine, the modeling of sustained triplet population by FEL radiatoin in photoexcited porphyrins, tissue optical property measurements, thermal effects of laser irradition on tissue, the near-ultraviolet effects on celluar tissue and the optical studies of protein flexibility using pulsed and modulated laser sources. Utilizing reverse-transforming techniques, a model system for porphyrin uptake and sequestration has been developed utilizing the commercially available porphyrin preparation, PHOTOFRIN II. Efficient photodynamical killing of the human promyelocytic leukemia HL-60 cell line under aerobic conditions after exposure to MC540 was demonstrated. A significant increase in the efficiency of HPD mediated photodynamical killing of a tumor by use pulse trained laser has occurred. Other than for skin and for blood, there appears to have been little systematic determination for the optical scattering and absorption coefficients of biological tissues. Measures are being taken to extend measurements of the scattering and absorption coefficients to a broad spectrum of major biological tissues. Histological and histochemical staining capabilities were developed for evaluating the tissue and cellular and subcellular level of thermally induced damage upon absorption of laser light. The tissue models are of rat skin and liver. Keywords: Laser induced flouresence; Free electron laser; Pulsed lasers; medical equipment.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 14, 1987
- Accession Number
- ADA179182
Entities
People
- David Vandermuelen
- Don Nealon
- J. L. Matthews
- M. M. Judy
- Michael V. Dennis