The Role of Chemical Inhibition of Gap-Junctional Intercellular Communication in Toxicology
Abstract
To achieve the goal of understanding the basic mechanism of chemical inhibition of gap junctional communication (a method by which cells transfer chemical information) we have performed research on a) validation of the fluorescence recover after photobleaching technique to study the effect of neurotoxins on cell-cell communication in living rat and human brain cells in culture; b) development of a new in vitro assay to measure cell-cell communication in vitro, utilizing scrape-loading and transfer of fluorescent dyes; c) further validation of the metabolic cooperation assay to detect tumor promoters and neurotoxins; d) started experiments to study the possible role of protein kinase C in the mechanism of inhibiting cell-cell communication and e) the development of a new quantitative technique to measure cell communication using flow cytometry and scape-loading/dye transfer. The results described in this report have been communicated at several meetings. The abstracts are attached to the progress report. Reprints and preprints are also enclosed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 24, 1987
- Accession Number
- ADA179683
Entities
People
- James E. Trosko
Organizations
- Michigan State University