Cloning of Acetylcholinesterase Gene in a Microbial Vector.

Abstract

Techniques to distinguish between the activities of human acetylcholinesterase and pseudocholinesterase have been developed. Human neuroblastoma cell lines have been shown to synthesize human acetylcholinesterase and the level of enzyme within these cells can be induced by treatment of cells with sodium butyrate or dibutyryl cAMP. These cell lines should be suitable as a source of mRNA for the cloning of the human gene into E. coli. Keywords: Genetics; Cholinesterases.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Feb 15, 1983
Accession Number
ADA179819

Entities

People

  • William J. Harris

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Amino Acids
  • Blood
  • Blood Cells
  • Cell Line
  • Cells
  • Central Nervous System
  • Chemical Warfare Agents
  • Chemistry
  • Culture Techniques
  • Erythrocytes
  • Fish
  • Gel Electrophoresis
  • Genetic Engineering
  • Medical Personnel
  • Proteins
  • Sodium Compounds

Fields of Study

  • Biology

Readers

  • Cardiovascular Physiology
  • Molecular Genetics
  • Neurotoxicology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech