Development of a DNA-Based Method for Distinguishing the Malaria Vectors, Anopheles gambiae From Anopheles arabiensis.

Abstract

The Anopheles gambiae complex includes six morphologically identical species, two of which (A. gambiae and A. arabiensis) are the primary African malaria vectors today. Since two or more of the species are commonly sympatric, epidemiological studies to determine the involvement of each in malaria transmission have been difficult. This report describes our efforts to identify a DNA segment from A. gambiae which when used as a probe on Southern blots containing both A. gambiae and A. arabiensis DNA will reveal a restriction fragment length polymorphism. These diagnostic bands are consistently seen on blots of whole genomic DNA. The method can also be used on dried single mosquitoes or mosquito abdomens although the DNA extraction method currently used does not always yield sufficient DNA for the test. Keywords: Clones; Assaying; Culicidae; Autoradiography; Hybridization.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1986
Accession Number
ADA179862

Entities

People

  • Victoria Finnerty

Organizations

  • Emory University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biology
  • Biomedical Research
  • Chemical Compounds
  • Chemistry
  • Classification
  • Contracts
  • Deoxyribonucleic Acids
  • Extraction
  • Hybridization
  • Identification
  • Malaria
  • Materials
  • Molecular Weight
  • Recombinant Dna
  • Security
  • Sensitivity
  • Universities

Readers

  • Breast cancer cell signaling and growth regulation.
  • Vector-Borne Disease and Entomology