Human Immune Responses to Dengue Viruses.

Abstract

We have analyzed the interaction between the peripheral blood lymphocytes (PBL) of non-immune donors and dengue virus-infected cells, which results in interferon (IFN) production. Autologous monocytes or the Epstein-Barr virus transformed B lymyphoblastoid cell line (Raji cells) infected with dengue virus were used as IFN inducer cells. PBL produced IFN when cultured with dengue virus-infected cells. IFN was detected as early as 2 to 4 hours after exposure of PBL to dengue virus-infected cells. Dengue virus-infected cells treated with glutaraldehyde or paraformaldehyde, which produced no infectious dengue virus, also induced IFN. These results indicate that BPL produced IFN in response to dengue virus-infected cells and that the production of IFN by PBL is due to stimulation of PBL by dengue-virus-infected cells. The ability of dengue-infected cells to induce IFN correlated with the appearance of dengue antigens in infected cells. Characterization of IFN-producing PBL with monoclonal antibodies demonstrated that the IFN-producing cells were heterogeneous.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1986
Accession Number
ADA181652

Entities

People

  • Francis A. Ennis

Organizations

  • University of Massachusetts

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Antigens
  • Biological Factors
  • Biomedical Research
  • Blood
  • Cardiovascular System
  • Cell Line
  • Cells
  • Chemistry
  • Epidemiology
  • Immune System
  • Incubation
  • Interferon
  • Lymphatic System
  • Lymphocytes
  • Monocytes
  • Proteins

Fields of Study

  • Medicine

Readers

  • Immunology
  • Virology (or Medical Virology).