Human Immune Responses to Dengue Viruses.
Abstract
The purpose of this contract was to analyze the immune responses to dengue infections. During the past few years we have focused on three immune responses; lysis of dengue virus-infected cells by peripheral blood mononuclear cells (PBMC) of non-immune donors; antibody-dependent complement-mediated lysis of dengue virus-infected cells; and interferon induction from PBMC by dengue virus. Results indicate that both PBMC-mediated cytotoxicity and antibody-dependent cell-mediated cytotoxicity (ADCC) can cause significant lysis of dengue-infected Raji cells; The PBL (Peripheral Blood Lymphocytes) that are active in lysing dengue virus-infected cells are heterogeneous and are contained in Leu11+ and T3+ susets. Results also indicated that PBL produced IFN in response on dengue virus-infected cells and that the production of IFN by PBL is due to stimulation of PBL by dengue virus-infected cells. The ability of dengue in infected cells. Treatment of monocytes with IFN decreased the yield of infectious virus more than 99% and the percentage of dengue-antigen positive cells by 98%. These results suggest that IFNs produced by PBL in response to dengue virus-infected cells may have an important role in controlling dengue infection and in the pathogenesis of dengue hemorrhagic fever and shock syndrome.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1986
- Accession Number
- ADA181718
Entities
People
- Francis A. Ennis
Organizations
- University of Massachusetts