Development of General Antisera for Trichothecenes

Abstract

Antibodies with narrow structural requirements for binding are useful in specific assays. Antibodies which bind a variety of structurally related compounds could be used in detection of classes of compounds. Therefore, we began exploring the relationship between immunogen (hapten) structure and the selectivity of resulting antibodies to various of the trichothecenes. We synthesized immunogens, used them to immunize rabbits and examined the polyclonal sera for their selectivity for various trichothecene compounds before preparing monoclonal antibodies. Keywords: Mycotoxins; Synthesis; Radioisotope; Immunoassay; EIA(Enzyme ImmunoAssay); Antiserum; Anguidine.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Feb 28, 1987
Accession Number
ADA182519

Entities

People

  • C. E. Cook
  • Carol C. Whisnant
  • Mansukh C. Wani

Organizations

  • RTI International

Tags

DTIC Thesaurus Topics

  • Acetic Acid
  • Aluminum Hydride
  • Biomedical And Dental Materials
  • Blood
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Detection
  • Freund'S Adjuvant
  • Fungi
  • Lymphatic System
  • Organic Chemistry
  • Plastic Explosives
  • Polymer Chemistry
  • Polymeric Films
  • Spectra

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Mycotoxin ecology in Amazonian ecosystems.