Regulation of Brain Neuropeptide Secretion by Lymphokines.

Abstract

Preliminary experiments outlined in the original application suggested that supernatants from mononuclear cell cultures stimulated with staphylococcal antigens or IL-2 could release SRIF from dispersed cortical neurons. Such activated supernatants are known to contain many cytokines, including interleukins-1 (IL-1). We elected to begin the study of this release activity by examining the effects of IL-1 and IL-2 in more detail, using pure products of recombinant DNA origin. Human recombinant interleukin-1-beta (rIL-1B) was tested in both cortical and diencephalic cells. Modest SRIF release activity was observed only at a concentration of 10- M. To investigate the effect of chronic IL-1 exposure, cultures were incubated for 48 hours with various concentrations of rIL-1B and the cells and media assayed for SRIF. Keywords: Somatostatin.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1987
Accession Number
ADA182564

Entities

People

  • Charles Dinarello
  • David Scarborough
  • James Meir
  • Seymour Reichlin

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Blood
  • Cells
  • Chemistry
  • Classification
  • Culture Techniques
  • Hospitals
  • Military Research
  • New England
  • Peptide Growth Factors
  • Peptides
  • Pituitary And Hypothalamic Hormones And Analogues
  • Procurement
  • Recombinant Dna
  • Regulations
  • Secretion
  • Security

Fields of Study

  • Medicine

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Immunology
  • Neuroscience