The Primary Structure of Acetylcholinesterase and Selective Antibodies for the Detection of Organophosphate Toxicity.

Abstract

The proposed work has been directed to determination of the structure of acetylcholinesterase. During the project period we have determined the primary structure of the Torpedo enzyme through amino acid sequencing and the isolation and sequencing of a c-DNA clone encoding for the 11S form of the enzyme. Peptides corresponding to the active center of the enzyme and a C-terminal region have been synthesized and antibodies are being raised for the purpose of detecting the phosphorylated enzyme and delineating functional regions of the molecule. Finally, progress has been made on the secondary structure of the enzyme. Keywords: Biochemistry.

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Document Details

Document Type
Technical Report
Publication Date
Dec 03, 1985
Accession Number
ADA182953

Entities

People

  • Palmer W. Taylor

Organizations

  • University of California, San Diego

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Acids
  • Amino Acids
  • Animals
  • Antibodies
  • Biomedical Research
  • Chemical Synthesis
  • Chemistry
  • Coding
  • Contracts
  • Dna Sequence Analysis
  • Genetic Code
  • Liquid Chromatography
  • Molecules
  • Protein Sequence Analysis
  • Proteins
  • Sequence Analysis

Readers

  • Molecular Genetics
  • Molecular and Cellular Biochemistry
  • Neurotoxicology