The Effects of Hydrazine and Related Compounds on Calcium-Calmodulin Regulated Synaptic Processes

Abstract

Results from this research effort have provided insights into the effects of hydrazines on calcium calmodulin regulated synaptic processes. The effects of hydrazines on the polarization-dependent calcium uptake into synaptazomes was studied. Initial studies performed by others suggested that hydrazines alter the depolarization-dependent calcium uptake by synaptozomes. After a detailed study to confirm these results and to look at the molecular mechanisms involved, we could not reproduce these reported effects of hydrazine on calcium fluxes. Hydrazine in concentrations as high as 100 micromolar did not have any significant effect on potassium stimulated calcium uptake into synaptazomes. Thus, our conclusion was that under physiological conditions used to study transmitter release, calcium uptake and protein phosphorylation in our laboratory, we did not see hydrazine effecting the calcium entry cross the membrane. Keywords: Neuronal excitability, Hydrazine, Calcium, Calmodulin kinase, Cytoskeleton.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 10, 1986
Accession Number
ADA183309

Entities

People

  • Robert J. Delorenzo

Organizations

  • Yale University

Tags

Communities of Interest

  • Weapons Technologies

DTIC Thesaurus Topics

  • Air Force
  • Amino Acids
  • Anticonvulsants
  • Biological Sciences
  • Carrier Proteins
  • Chemistry
  • Depolarization
  • Intact Stability
  • Membranes
  • Molecular Weight
  • Nervous System
  • Pharmacology
  • Proteins
  • Standards
  • Synapses
  • Terminals
  • Tissues

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Neuroscience
  • Underwater engineering and Marine Technology.