Evolution of Phosphofructokinase.

Abstract

Comparative sequence data suggest that eucaryotic phosphofructokinase (PFK) has evolved from a procaryotic precursor by gene duplication, fusion, and mutation of previous catalytic sites into new regulatory ligand binding sites. We are attempting to duplicate these processes by recombinant DNA technology. The E. coli pfk gene is being recloned into a convenient plasmid. Using a synthetic oligonucleotide that matches the mammalian link region, two E. coli pfk genes will be joined. The properties of the expressed duplex enzyme will be analyzed. Utilizing site-directed mutagenesis, conversion of one of the two catalytic sites to a regulatory site will be carried out. Keywords: Protein evolution, Protein engineering.

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Document Details

Document Type
Technical Report
Publication Date
Jul 31, 1987
Accession Number
ADA183321

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People

  • Robert G. Kemp

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DTIC Thesaurus Topics

  • Biochemistry
  • Biological Sciences
  • Chemistry
  • Classification
  • Deoxyribonucleic Acids
  • Engineering
  • Military Research
  • Molecular Biology
  • Mutations
  • Personal Information Managers
  • Production
  • Protein Engineering
  • Protein Sequence Analysis
  • Recombinant Dna
  • Security
  • Sequences

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  • Biology

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  • Molecular Genetics

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  • Biotechnology