Processing of Archaebacterial Intron-Containing tRNA Gene Transcripts.

Abstract

An essay for the Halobacterium volcanii tRNATrp intron endonuclease has been developed. This enzyme accurately cleaves the H. volcanii intron containing tRNATrp substrates, but does not act on a typical eukaryotic intron-containing precursor, yeast pretRNAPhe. Substrate deletion analysis indicates that mature tRNA structures and intron sequences are not absolutely required for recognition and processing by this enzyme. These findings suggest a sequence specific recognition pattern. An activity capable of removing 5' flanking sequences (RNAase P) from intron and non-intron containing pretRNAs has also been partially purified from this organism and techniques have been developed to investigate this activity in Thermoplasma. We are also investigating the overall distribution of introns in H. volcanii tRNA genes. Using the technique of orthogonal field gel electrophoresis two large BamH1 restriction fragments which contain the majority of tRNA genes in this organism have been identified. Keywords: tRNA, DNA sequencing.

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Document Details

Document Type
Technical Report
Publication Date
Jul 31, 1987
Accession Number
ADA183646

Entities

People

  • Charles J. Daniels

Organizations

  • Ohio State University

Tags

Communities of Interest

  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Archaea
  • Availability
  • Chemistry
  • Classification
  • Dna Sequence Analysis
  • Electrophoresis
  • Gel Electrophoresis
  • Identification
  • Nucleotides
  • Precursors
  • Recognition
  • Ribonucleic Acids
  • Security
  • Sequence Analysis
  • Sequences
  • Substrates
  • Trna

Fields of Study

  • Biology

Readers

  • Molecular Genetics