The Quantitation of Pyridostigmine in Plasma by HPLC (High Pressure Liquid Chromatographic).

Abstract

A normal-phase HPLC method utilizing ion-pair extraction for the quantitation of pyridostigmine in plasma is described. The limit of quantitation was 2 ng/ml plasma; intra-day precision with coefficient of variation (CV) of 1.4% (50 ng/ml) and inter-day precision of 2.6% (50 ng/ml) were obtained. The method was designed for use in pharmacokinetic studies with the Rhesus monkey, but has been successfully adapted and applied to the analysis of plasma from rats dosed with pyridostigmine in the feed. Keywords: Blood plasma; Acetycholinesterase, Pharmicokinetics.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1987
Accession Number
ADA183795

Entities

People

  • Bert Ho
  • John R. Ryabik

Organizations

  • Letterman Army Hospital

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetonitrile
  • Acids
  • Animals
  • Chlorides
  • Classification
  • Detection
  • Laboratory Animals
  • Linear Regression Analysis
  • Materials
  • Nitriles
  • Organic Materials
  • Precision
  • Pyridostigmine Bromide
  • Regression Analysis
  • Rhesus Monkeys
  • Toxicology
  • United States Government

Fields of Study

  • Chemistry

Readers

  • Analytical Chemistry
  • Neurotoxicology