Comparative Infectivity Determination of Candidate Live Dengue Virus Vaccine in Monkeys, Mosquitoes and Cell Cultures.
Abstract
The following live, attenuated dengue virus vaccines strains were tested for their respective immunogonicity in rhesus monkeys: dengue-1 (TP79-56, code TSI-GSD216 and 45AZ-5, code No. TSI-GSD214; dengue 3 (CH53489, clone 24/28); and dengue-4 (341750 Carib). It was found that 417 MID50 (mosquito infectious dose 50) and 116 MID50 were required for seroconversion of 50% of the animals with the 45AZ5 and the TP79-56 vaccine, respectively. Upon challenge with the homologous wild type virus, dengue-1 immune animals showed a typical secondary antibody response. A dose of 2.9 MID50 (dengue-3 vaccine) and of 0.158 MID50 (parent dengue-3 virus) seroconverted 50% of the animals. Experimental dat showed that the DEN-4 vaccine was highly attenuated: a dose of 60 MID50 of the parent, wild type dengue-4 virus (341750 Carib) was required to elicit seroconversion of 50% of the experimentl animals. The dengue-4 vaccine may be suitable for trials with human volunteers.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 27, 1987
- Accession Number
- ADA183804
Entities
People
- Edmundo Kraiselburd
Organizations
- University of Puerto Rico