Bimane Derivatives as Fluorescent Probes for Biological Macromolecules.
Abstract
Syn-9, 10-Dixoabimanes (1,5-diazabicyclo3.3.0octa-3,6-diene-2,8-diones) are photostable, highly fluorescent compounds which can be attached to biological macromolecules as fluorescent labels. Syntheses of the important new intermediates for labeling agents, syn-tricyclic bimanes, RuCn (R=CH3 or C1) and n= 0.1,2,3 have been developed. The conversion of the compounds with n=2 into a diene has been accomplished, successfully raising the adsorption coefficient. However, interesting and surprising physical behavior conspired to drastically diminish the fluorescence quantum yield. The discovery of several routes to unsymmetrically substituted bimanes prepared by nucleophilic displacement on bromoalkyl derivatives has revealed new classes of labeling agents for macromolecules. These include a cross-linking agent.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 18, 1987
- Accession Number
- ADA184048
Entities
People
- Edward M. Kosower
Organizations
- Tel Aviv University