Monoclonal Antibodies that Recognize Proteins Unique to Somatic Embryos of Daucus carota.

Abstract

We have developed a panel of monoclonal antibodies (Mabs) to several antigens of Daucus carota somatic embryos. We prepared over 2400 hybridoma cultures from splenocytes of Balb/c mice immunized with lyophilized somatic embryo cells. The MAbs were screened on embryo, callus cell, and chloroplast extracts using an automated sampling system and computer-assisted analyses. Most of the MAbs reacted with antigens common to embryo and callus cells, nut MAbs from al least 5 hybridoma lines recognized single antigens unique to the somatic embryos. Other lines produced antibodies to chloroplast antigens, including the light-harvesting chlorophyll protein (LHCP).

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1985
Accession Number
ADA184180

Entities

People

  • Alexander E. Karu
  • J. A. Smith
  • Jin Joo Choi
  • M. Krauss
  • Z. R. Sung

Organizations

  • University of California

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  • Antibodies
  • California
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Fields of Study

  • Biology

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  • Immunology
  • Molecular and genetic basis of cancer.