The Microbial Degradation of TCE (Trichloroethylene).

Abstract

The objective of this research was to characterize naturally occurring microorganisms capable of degrading trichloroethylene (TCE). Microorganisms tolerant to TCE were isolated from an aeration tower at Wurtsmith AFB MI and from industrial sludges at Hill AFB UT. Isolates from the Wurtsmith samples exhibited the highest density of cells with TCE as the only apparent organic carbon source and were selected as the inoculum for degradation studies. However, the capability of selected microorganisms to degrade TCE was not substantiated in both gas chromatography analyses and samples inoculated in culture bottles. Experiments using radiolabeled carbon-14 to study assimilation suggest that TCE is metabolized into a polar form by the microorganism. Subsequent cultures grown in radiolabeled TCE did not duplicate the previous results possible because of contamination, selection of variants or loss of culture. The organism responsible or the apparent TCE degradation may belong in the genus Citrobacter.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1987
Accession Number
ADA184884

Entities

People

  • J. H. Wolfram
  • J. T. Case
  • R. D. Rogers

Tags

Communities of Interest

  • Energy and Power Technologies
  • Ground and Sea Platforms

DTIC Thesaurus Topics

  • Air Force
  • Air Force Facilities
  • Alcohols
  • Bacteria
  • Biodegradation
  • Chemistry
  • Chromatography
  • Ecology
  • Engineering
  • Environment
  • Environmental Protection
  • Gas Chromatography
  • Materials
  • Measurement
  • Metabolism
  • Microorganisms
  • Organic Compounds

Fields of Study

  • Environmental science

Readers

  • Environmental Impact Assessment (EIA) of Proposed Air Force Base Actions.
  • Microbial Pathology
  • Polymer Science and Engineering.

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation