Testing Experimental Compounds against Leishmaniansis in Laboratory Animal Model Systems

Abstract

Pentostam Dose Responses for L. donovani, L. m. mexicana, and L. b. guyanensis have been determined in BALB/c mice using amastigotes (A), primary culture promastigotes (PCP), and subcultured promastigotes (SP). In each test system, PCP are more resistant to treatment than are A or SP. This is biologically significant, since PCP are equivalent to the ineffective stage from sandflies. The data also show that Pentostam causes improvement and parasitological cures in WRAIR primary (hamster), secondary (BALB/c), and tertiary (owl monkey) test systems at doses and regimes equivalent to those used to treat humans. The validity of each of these models in drug screening to predict the outcome in human infections is established. Seven of 10 WRAIR 1984 compounds suppress L. donovani 79 to 94% at the MTD< MTD/2, and MTD/4. It is highly probable they will be competitive with Pentostam.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1984
Accession Number
ADA184922

Entities

People

  • Jan S. Keithly

Organizations

  • Cornell University

Tags

DTIC Thesaurus Topics

  • Cells
  • Chemical Synthesis
  • Chemistry
  • Health Services
  • Laboratory Animals
  • Medical Personnel
  • Organic Chemistry

Fields of Study

  • Biology

Readers

  • Distributed Systems and Data Platform Development
  • Infectious Disease/Epidemiology
  • Parasitology and Pharmacology of Malaria.