Rapid Diagnosis of Arbovirus and Arenavirus Infections by Immunofluorescence.

Abstract

Spot-slides bearing cells infected with arboviruses were supplied to USAMRIID for immunofluorescence tests. A safe and quick ELISA for Crimean-Congo hemorrhagic fever (CCHF) virus was developed using infected, formalin-fixed CER cells as antigen. A retrospective serologic survey of equatorial Africa for antibodies in man to CCHF, Rift Valley fever (RVF), Ebola, Lassa fever, and Marburg viruses was done. In Sudan, antibodies to Marburg, CCHF, Lassa, RVF, and Ebola viruses were detected in prevalences between 0.2-11.2% with geographic clustering especially in the southern and central provinces. In Senegal, antibodies to Ebola, RVF and CCHF viruses were recorded in prevalences from 5-14%. In Ethiopia, some sera from a putative yellow fever outbreak of 1960-62 were positive to Ebola virus. Ebola IgM was detected in one patient suggesting that Ebola was active in the 1960's. Keywords: Arboviruses, Arenaviuses, Immunofluorescence, ELISA, Spot-slides, Arboviruses, Hemorrhagic fever, Serosurvey, Equational Africa.

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Document Details

Document Type
Technical Report
Publication Date
Dec 31, 1984
Accession Number
ADA185464

Entities

People

  • Gregory H. Tignor

Organizations

  • Yale University

Tags

DTIC Thesaurus Topics

  • Africa
  • Arboviruses
  • Army Personnel
  • Detection
  • Drainage Basins
  • Geographic Regions
  • Health Services
  • Lassa Fever
  • Light Sources
  • Marburg Virus
  • Medical Personnel
  • Rift Valley Fever
  • Rodents
  • Viral Hemorrhagic Fevers
  • Virus Diseases
  • Viruses
  • Yellow Fever

Fields of Study

  • Biology

Readers

  • Virology (or Medical Virology).