Effects of Hydrazines upon Cyclic Nucleotide Regulated Neuronal Processes
Abstract
The funded project was designed, initially to explore the effects of hydrozines upon cyclic nucleotide regulated neuronal processes. Cyclase as it was discovered that hydrazines were potent activators of this enzyme. In order to understand hydrazine actions in the CNS, it was required that more basic knowledge of the adenylate cyclase cascade by accumulated and this study probed some of the distinctions between neural and non-neural adenylate cyclase with that in mind. Specifically, the following has been accomplished during the project period: Interactions between the cytoskeleton and synaptic membrane adenylate cyclase have been probed and we have found a reversible attachment between the GTP-binding proteins regulating adenylate cyclase and that membrane. We have discovered that GTP binding proteins directly interact and may exchange nucleotide with one another, and have hypothesized this mechanism as an intracellular regulator of signal transduction. We have discovered a novel, neural GTP binding protein and are in the process of purification and characterization. We have devised a method for measuring adenylate cyclase in monolayers of permeable cells and have used this method to explore the coupling between receptors and adenylate cyclase GTP-binding proteins. It is hoped that an increased understanding of the neuronal adenylate cyclase system will lead to an increased understanding of the effects of certain neurotoxins, and to the design of strategies to prevent and/or treat the effects of those compounds.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 30, 1987
- Accession Number
- ADA185711
Entities
People
- Mark M. Rasenick
Organizations
- University of Illinois at Chicago