Purified Mojave Toxin Interacts with CA++Mg++ ATPase in Rat Synaptic Membranes.

Abstract

Calcium mediated channels produce changes in intracellular calcium concentration in neural, skeletal, and cardiac tissues. The Ca Mg ATPase is the enzyme responsible for maintaining the Ca gradient across the membrane. Studies of these Ca dependent membrane proteins have been limited due to the scarcity of naturally occuring toxins with which to probe the systems. In this report, we describe the actions of Mojave toxin, an acidic toxin isolated from the venom of Crotalus scutulatus scutulatus, on the Ca Mg ATPase and its associated dihydropyridine receptor sites. Mojave toxin induced an increase in rat brain synaptic membrane Ca++Mg++ ATPase activity and inhibited dihydropyridine binding. These data suggest that Mojave toxin may be a useful probe of the Ca channel complex.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1987
Accession Number
ADA186824

Entities

People

  • James J. Valdes
  • James P. Chambers
  • Matthew J. Wayner

Organizations

  • University of Texas at San Antonio

Tags

DTIC Thesaurus Topics

  • Animals
  • Biochemistry
  • Biological Sciences
  • Cell Membrane
  • Cells
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Classification
  • Engineering
  • Laboratory Animals
  • Materials
  • Membranes
  • Proteins
  • Proteomics
  • Synapses
  • Tissues

Fields of Study

  • Biology
  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Superconducting Magnet Technology