Uptake of T-2 Mycotoxin in Cultured Cells. Relationship to Sodium Fluoride and Cell Type
Abstract
We examined the effect of sodium fluoride on uptake of tritium- labeled T-2 toxin (molecules/cell) in Chinese hamster ovary (CHO) and African green monkey kidney (VERO) cells. Correlations were made to temperature and toxin concentration over time. As expected, toxin uptake increased in both cell types with increasing time and temperature. Under all parameters, VERO cells exhibited significant increases in the rate of toxin uptake. The rate of equilibrium was affected by both temperature and sodium fluoride. Statistical analysis of individual time points along the curve demonstrated that sodium fluoride significantly increased cell-associated toxin at most time points. Analysis of the slopes of uptake curves from 0 to 20 min indicated significant (p<0.05) differences in the rates of T-2 uptake in both cell types and toxin doses in the presence of sodium fluoride. The increase in toxin uptake in the presence of sodium fluoride was not due to altered cell membrane permeability caused by sodium fluoride. Difference in total uptake between cell types may reflect cell specific factors such as the number of intracellular binding sites. This study demonstrates that sodium fluoride significantly increases cell- associated T-2 toxin and the rate of toxin uptake in two cultured cell lines. Of the various actions of sodium fluoride, inhibition of ATP produced by glycolysis may be the most important. Sodium fluoride induced inhibition of glycolytic energy production may lead to increased cellular uptake of T-2, decreased cellular export of T-2, or a combination of both events.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 20, 1986
- Accession Number
- ADA187062
Entities
People
- Lee J. Martin
- Lynn R. Trusal
Organizations
- United States Army Medical Research Institute of Infectious Diseases