Genetic and Physiological Studies of Bacillus anthracis Related to Development of an Improved Vaccine
Abstract
The primary objective of the research is to gain information and to develop genetic systems that will contribute to development of an improved vaccine for anthrax. During the year represented by this report our research concentrated largely on (i) characterization of the conjugative plasmid, pLS20, of B. subtilis and its ability to transfer plasmids among B. subtilis, B. cereus, B. thuringiensis, and B. anthracis; (ii) physical and genetic characterization of the temperate B. thuringiensis phage, TP21, which is active on B. anthracis and whose prophage exists as a plasmid; (iii) further physical and genetic characterization of the B. thuringiensis conjugative plasmids pX011 and pX012; (iv) the mechanism of transfer of the B. anthracis plasmids, pX01 and pX02, by the conjugative plasmid pX012; and (v) transformation of B. cereus and B. anthracis with plasmid DNA. Keywords: Bacillus anthracis, B. anthracis plasmids, Bacillus conjugative plasmids, Anthrax protective antigen, Anthrax toxin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1987
- Accession Number
- ADA187381
Entities
People
- Curtis B. Thorne
Organizations
- University of Massachusetts Amherst