Mass Spectral Investigations on Toxins. 7. Detection and Accurate Quantitation of Picogram Quantities of Macrocyclic Trichothecenes in Brazilian Plant Samples by Direct Chemical Ionization-Mass Spectrometer/Mass Spectrometer Techniques
Abstract
Roridins and baccharinoids are macrocyclic trichothecenes. They formed (M+NH4)(+) adducts effectively with ammonia under chemical ionization conditions. The daughter spectra of these adducts were characteristic of the macrocyclic ester bridges and indicate several common neutral losses. Experiments designed for the simultaneous detection of these macrocyclic trichothecenes by sequentially monitoring their characteristic daughter ions and the corresponding parent spectra were found to be applicable for the analysis of Brazilian Baccharis plants. The compounds detected in these samples were accurately quantified using a synthetically modified macrocyclic trichothecene, 8-ketoverrucarin A, as the internal standard. The minimum detectable limits of roridins and baccharinoids under various experimental conditions used for their detection and quantification were measured to be 10-100 pg. Keywords: Trichothecenes, Macrocyclic trichothecenes, Roridins, Baccharinoids.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1987
- Accession Number
- ADA187481
Entities
People
- Emory W. Sarver
- Thaiya Krishnamurthy