Application of New Technologies to Radiation Biodosimetry in Mammalian Systems. Volume 2.
Abstract
Various procedures, including flow cytometry analysis, premature chromosome condensation (PCC) techniques, deoxyribonucleic acid (DNA) damage and repair kinetics, amd high resolution microscopic image analysis (Cell Analyzer System), have been used to determine radiation damage produced by low doses of 137Cs. Peripheral lymphocytes isolated from whole-body irradiated mice were stained with Ig and Thy-1.2 immunofluorescence antibodies and analyzed with cytometry to determine the cell number and changes in subpopulations (T- and B-cells) as a function of postirradiation time. The results indicated a dose-dependent decrease of lymphocyte numbers during the period of 5-day postirradiation time. Cell survival curves constructed from different subpopulations of lymphocytes in the dose range of 0-2 Gy showed a linear relationship between radiation dose and log surviving cell number in different subpopulations of lymphocytes, indicating the radiation sensitive nature of the lymphocytes. The DNA damage and repair kinetics were measured from in vitro and in vivo mouse lymphocytes, Chinese hamster ovary (CHO) cells, and human squamous carcinoma A431 cells to compare the cellular radiosensitivity with DNA repair capacity. Quantitativity similar amounts of initial DNA damage were produced among the different kinds of cells. However, the repair of DNA strand breaks was significantly faster in A431 cells than CHO and lymphocytes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1987
- Accession Number
- ADA187498
Entities
People
- Edith M. Lord
- Peter C. Keng
- Robert M. Sutherland
Organizations
- University of Rochester