Degradation of Albumin by Trypsin: Potential Application of High-Performance Liquid Chromatography (HPLC) and Differential Scanning Calorimetry (DSC) for Monitoring the Degradation of Proteinaceous Toxins by Proteolytic Enzymes.

Abstract

Albumin was selected as a model protein for developing assay systems for monitoring degradation by proteolytic enzymes. Using a diode array detector, high-performance liquid chromatography was ideal for following albumin digestion by trypsin. The albumin peak gradually diminished and was replaced by several peaks corresponding to shorter peptides. In contrast, differential scanning calorimetry was of no value in following the disappearances of the protein. A thermal transition resulting from some confirmational change occurred at 52 C but did not change upon digestion of the protein.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1987
Accession Number
ADA188171

Entities

People

  • Ilya Elashvilli
  • Penelope Diamandaras
  • William E. White

Organizations

  • Edgewood Chemical Biological Center

Tags

DTIC Thesaurus Topics

  • Albumins
  • Amino Acids
  • Calorimetry
  • Chemical Synthesis
  • Chemistry
  • Chromatography
  • Classification
  • Degradation
  • Detectors
  • Digestive System Processes
  • Engineering
  • Liquid Chromatography
  • Mixtures
  • Monitoring
  • Scanning
  • Security
  • Transitions

Readers

  • Analytical Chemistry
  • Materials Science and Engineering.
  • Molecular and Cellular Biochemistry