Multicomponent Analysis Using Synchronous-Excitation Phase-Resolved Fluorescence Spectroscopy.

Abstract

Phase resolved fluorescence spectroscopy was used to incorporate fluorescence lifetime selectivity into multicomponent fluorimetric determinations. Quantitative analysis was demonstrated for systems of up to six fluorescent components with overlapping spectra. Multidimensional data formats of phase resolved fluorescence intensity as a function of spectral and lifetime parameters were developed, including formats for both synchronous excitation and total luminescence spectroscopy. It was shown that phase resolution improves quantitative results significantly over the use of steady-state, non-lifetime dependent intensity measurements. Specific systems studied include polycyclic aromatic hydrocarbons and metal chelates. Sodium taurocholate micelles were studied as a means by which synergistic interactions between fluorescence molecules in a sample could be minimized. Very interesting results were obtained, and further investigations of the micellar systems are being conducted. Keywords: Fluorescence spectroscopy, Chemical analysis, Phase resolved, Fluorescence spectroscopy, Synchronous excitation, Total luminescence spectroscopy, Time resolved fluorescence.

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Document Details

Document Type
Technical Report
Publication Date
Dec 04, 1987
Accession Number
ADA188883

Entities

People

  • Linda B. Mcgown

Organizations

  • Oklahoma State University–Stillwater

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Aromatic Hydrocarbons
  • Chemical Analysis
  • Chemistry
  • Cyclic Hydrocarbons
  • Detectors
  • Excitation
  • Frequency
  • Identification
  • Intensity
  • Luminescence
  • Military Research
  • Modulation
  • Pattern Recognition
  • Phase Modulation
  • Recognition
  • Spectroscopy
  • Steady State

Readers

  • Chemistry (specifically Chemical Fluorescence)
  • Image Processing and Computer Vision.
  • Polymer Science and Technology