Immunological Analysis of Cell-Associated Antigens of Bacillus anthracis,
Abstract
By using electrophoretic immuno-transblots, EITB (Western blots), we compared sera from Hartley guinea pigs vaccinated with a veterinary live-spore anthrax vaccine to those vaccinated with the human anthrax vaccine, consisting of aluminum hydroxide-absorbed culture proteins of Baccillus anthracis strain V770-NP-1R. Sera from animals vaccinated with the spore vaccine recognized two major B. anthracis vegetative-cell-associated proteins not recognized by sera from animals receiving the human vaccine. These proteins, termed extractable antigens 1 (EA1) and 2 (EA2), have molecular masses of 91 and 62 kilodaltons, respectively. The EA1 protein appeared to be coded by chromosomal DNA, whereas the EA2 protein appeared to be coded by chromosomal DNA, whereas the EA2 protein was only detected in strains possessing the pX01 toxin plasmid. Both of the EA proteins were serologically distinct from the three anthrax toxin components, as determined by monoclonal antibody to protective antigen, edema factor, and lethal factor, and specific antisera to the EA proteins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 17, 1986
- Accession Number
- ADA189584
Entities
People
- John W. Ezzell Jr.
- Teresa G. Abshire
Organizations
- United States Army Medical Research Institute of Infectious Diseases