Procedures for Detoxification of Brevetoxins PbTx-2 and PbTx-3 (Neurotoxins from the Florida Red Tide Dinoflagellate Ptychodiscus brevis)
Abstract
Procedures for(detoxification of the brevetoxins PbTx-2 and PbTx-3 were investigated using the Japanese madaka (Oryzias latipes) as the assay model. PbTx-2 was 100% lethal to these fish at concentrations above 70 ng/ml in the aquarium water, while PbTx-3 was less potent. Treatment with 0.5 ml 0.1 N naOH for 10 min detoxified a lethal dose of either PbTx-3 or PbTx-3 to below the detection limits of this assay (<10 ng/ml). Decreasing NaOH concentration required correspondingly longer incubation times. Potency of both toxins was also destroyed by incubation at 500 C for 10-15 min. Steam autoclaving at 122 C (30 min, 18 psi) was not sufficient to detoxify brevetoxins. These results show that washing or soaking in a dilute NaOH solution will decontaminate laboratory glassware and equipment and render it safe for normal handling. Disposable waste can be either soaked in a NaOH solution prior to disposal or burned in an incinerator with a combustion chamber of at least 500 C. Steam autoclaving, however, is not a viable method of decontamination.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 10, 1987
- Accession Number
- ADA190155
Entities
People
- Mark A. Poli
Organizations
- United States Army Medical Research Institute of Infectious Diseases