Molecular Toxicology of Chromatin.
Abstract
Part I. Benzamide-DNA Interactions; Deductions from Binding, Enzyme-Kinetics and from X-ray Structural Analysis of a 9-Ethyladenine-Benzamide Adduct: The interaction of benzamide with the isolated components of calf thymus poly(ADP-ribose) polymerase and with liver nuclei has been investigated. Part II. Molecular Interactions Between DNA, Poly(ADP-Ribose) Polymerase and Histones: Molecular interactions between purified poly(ADP-ribose) polymerase, whole thymus histones, histone H1, rat fibroblast genomic DNA, and closed circular and linearized SV40 DNA were determined by the nitrocellulose filter binding technique. Binding of the polymerase protein, or histones, to DNA was greatly augmented when both enzyme protein and histones were present simultaneously. Part III. Analysis of the Molecular Contacts Between Poly(ADP-ribose) Polymerase and DNA: The interaction between poly (ADP-ribose) polymerase and various uniquely end labeled restriction fragments from SV40 an pBR322 DNAs was studied employing nuclease protection experiments. DNasel footprinting indicated that approximately 66-85 bp of DNA was protected by poly (ADP-ribose) polymerase from DNasel attack, and that a segment of DNA probably lies outside on the surface of the polymerase protein in the polymerase-DNA complex.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 31, 1987
- Accession Number
- ADA191557
Entities
People
- Ernest Kun
Organizations
- University of California, San Francisco