Chemical Synthesis Coxiella Burnetti Lipopolysaccharides: Structural Studies of Coxiella Burnetti Lipopolysaccharides.

Abstract

Lipopolysaccharide (LPS) materials were extracted from C. burnetii (CB9MI514 & CB9MIIC4) with a modified phenol-water procedure, purified by ultracentrifugation, and forwarded to this facility for structural characterization. The LPS was degraded by partial acid hydrolysis and separated into lipophilic and hydrophilic fractions with the designations, Lipid C and O-Antigen, respectively. Preliminary structural data has been obtained for Lipid C and the O-Antigen of CB9MI514 and more confirmatory data and corroborative evidence is provided for the structure of O-Antigen as well as new information on the Lipid C of CB9MIIC4. Sample permethylation on intact LPS (CB9MIIC4) by differential CD3/CH3 analysis has provided an approximation of molecular weight. Supercritical fluid chromatography (SFC) and SFC-MS studies have been initiated for application to this problem. Keywords: Toxicity; Immunology; Molecular structure.

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Document Details

Document Type
Technical Report
Publication Date
Nov 30, 1987
Accession Number
ADA192078

Entities

People

  • G. R. Her
  • V. N. Reinhold

Organizations

  • Harvard T.H. Chan School of Public Health

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biomedical Research
  • Carbohydrates
  • Chemical Synthesis
  • Chemistry
  • Chlorides
  • Chromatography
  • Health Services
  • Lipids
  • Lipopolysaccharides
  • Liquid Chromatography
  • Mass Spectra
  • Mass Spectrometry
  • Materials
  • Molecular Weight
  • Organic Chemistry
  • Q Fever
  • Spectrometry

Readers

  • Infectious Disease/Epidemiology
  • Microbial Pathology
  • Molecular and Cellular Biochemistry