Pathophysiology and Toxicokinetic Studies of Blue-Green Algae Intoxication in the Swine Model
Abstract
Neurotoxin production is being scaled up; purification methods are being compared. Mouse bioassay is used to identify toxic fractions. Stability studies with anatoxin-a(s) revealed toxin degradation, particularly in organic solvents; the process can be slowed by acidification. TLC has revealed previously unrecognized contaminants. We anticipate purification of toxin in its free base form, with normal phase HPLC; because of negligible UV absorption, new methods of detection are needed. Inhibition by anatoxin-a(s) of eel and human erythrocyte acetylcholinesterases was time and concentration dependent and followed first order kinetics, indicative of reversible binding followed by irreversible inhibition. RBC cholinesterase was more sensitive. Inhibition of horse serum cholinesterase, however, was non-linear.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 21, 1986
- Accession Number
- ADA192177
Entities
People
- Andrew M. Dahlem
- Val R. Beasley
- Wanda M. Haschek-hock
- Wayne W. Carmichael
- William O. Cook
Organizations
- University of Illinois Urbana–Champaign