In Vitro Metabolism of T-2 Mycotoxin 1,2
Abstract
In vitro metabolism of T-2 mycotoxin (T-2) was studied in Vero cells, rat spleen lymphocytes, chicken embryo heart cells, rat small intestinal segments, and rat liver hepatocytes. The method used was thin-layer chromatography (TLC) of (3H)T-2 and its metabolic products, followed by radioactive scanning of the plates. Vero cells, lymphocytes, and heart cells metabolized 5 to 35 percent of the T-2 to HT-2 mycotoxin (HT-2) after 24 hr exposure. No other metabolites were detected with these three cell systems. Rat intestinal segments, everted onto pipets, converted T-2 into three metabolites migrating in the range between T-2 tetraol and HT-2 on the TLC plates. Hepatocytes metabolized T-2 most rapidly, as indicated by complete disappearance of the parent compound within 4 hr. In addition to the T-2 peak, four predominant peaks appeared on the plates, one of them, increasing with time at the origin, was predominantly composed of glucuronide conjugates.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 10, 1986
- Accession Number
- ADA192647
Entities
People
- John C. O'brien
- Judith G. Pace
- William L. Thompson
Organizations
- United States Army Medical Research Institute of Infectious Diseases