Nucleotide Sequence of the Protective Antigen Gene of Bacillus Anthracis

Abstract

The DNA sequence of the protective antigen gene from Bacillus anthracis and the 5' and 3' flanking sequences were determined. Protective antigen is one of three proteins comprising anthrax toxin. The open reading frame is 2319 base pairs (bp) long, of which 2205 bp encode the 735 amino acids of the secreted protein. This region is preceded by 29 codons, which appear to encode a signal peptide having characteristics in common with those of other secreted proteins. A consensus TATAAT sequence was located at the putative -10 promoter site. Keywords: Protective antigen, Gene, Nucleotide sequence, Nucleic acids.

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Document Details

Document Type
Technical Report
Publication Date
Feb 02, 1988
Accession Number
ADA192648

Entities

People

  • F. Eden-mccutchan
  • J. R. Lowe
  • M. Vodkin
  • S. L. Welkos
  • S. M. Leppla

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Bacteria
  • Biological Toxins
  • Cells
  • Chemistry
  • Dna Sequence Analysis
  • Free Energy
  • Genetic Code
  • Infectious Diseases
  • Molecular Biology
  • Nucleic Acids
  • Nucleotides
  • Recombinant Dna
  • Ribonucleic Acids
  • Rodents
  • Sequence Analysis

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular Genetics

Technology Areas

  • Biotechnology