Structure and Function of Cytochrome P-450 Genes
Abstract
The cytochrome P450IIC2 (previously designated P-450PBc) subfamily of the phenobarbital-inducible family of cytochrome P-450 contains several closely related members. We have reported previously the characterization of complementary deoxyribonucleic acids for four of these P-450's, partial characterization of three of the corresponding genes and have shown that phenobarbital-induction can largely be accounted for by an increase in transcription rates. In year 3 we have continued our analysis of the genes of this subfamily concentrating on the 5' flanking regions which probably contain regulatory regions. To complement our previous characterization of the 3' end of the P450IIC3 gene we have isolated a lambda phage clone containing the 5' portion of the gene. This cloned genomic fragment contained exons 2-6 and combined with the earlier work defines the structure of exons 2-9 into the 3' flanking region spanning more than 25 kilobase pairs. We are continuing our search for a cloned genomic fragment containing exon 1 and the 5' flanking region. We have also characterized a genomic fragment containing exons 1-5 of the phenobarbital-in-ducible P450IIC4 gene. We are presently continuing the isolation and characterization of the genes and have begun to study the effect of phenobarbital on the chromatin structure of these genes and to characterize nuclear proteins that bind to the genes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 25, 1988
- Accession Number
- ADA192750
Entities
People
- Byron Kemper
Organizations
- University of Illinois Urbana–Champaign