Isolation of Genomic Clone for Human Cholinesterase.

Abstract

The goal of this work is to isolate the gene for human serum cholinesterase (EC 3.1.1.8). We have succeeded in isolating a partial clone that codes for amino acids phenylalanine 195 through leucine 574, and contains 302 bp of the 3' untranslated region. This clone was found by screening a human brain Complementary Deoxyribonucleic Acid (cDNA) library with oligonucleotide probes. The probes corresponded to the amino sequence of human serum cholinesterase. The coding region of our 1440 bp clone matched the amino acid sequence of human serum cholinesterase perfectly. This suggested that human cholinesterase was identical in brain and in serum. We are continuing to search the brain cDNA library for other cDNA clones that will extend our present clone in the 5' direction so as to have the complete coding region of cholinesterase. Keywords: Pseudocholinesterase, Genetic engineering, Recombinant DNA, Gene sequencing.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1986
Accession Number
ADA192751

Entities

People

  • Oksana Lockridge

Organizations

  • University of Michigan

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical Research
  • Blood
  • Blood Coagulation
  • Blood Coagulation Factors
  • Brain
  • Cells
  • Chemical Warfare Agents
  • Chemistry
  • Computer Programming
  • Deoxyribonucleic Acids
  • Dna Sequence Analysis
  • Genetic Structures
  • Medical Personnel
  • Nucleic Acids
  • Nucleotides
  • Recombinant Dna

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Neurotoxicology

Technology Areas

  • Biotechnology