The Rhesus Monkey: A Primate Model for Hemopoietic Stem Cell Studies

Abstract

Two heterogeneous cell populations (CP 1-7 and CP 8-10) were separated from rhesus monkey bone marrow using counterflow centrifugation elutriation (CCE). These two cell populations were distinct with respect to morphological composition, expression of cell surface antigens. hemopoietic progenitor cell activity, and concentration stem cells (HSC). The hemopoietic progenitor cell activity and HSC were concentrated in CP 8-10. In autologous transplantation studies, CP 8-10 reconstituted the lymphohemopoietic system of lethally irradiated monkeys in a manner similar to that of monkeys transplanted with unfractionated bone marrow cells. CP 1-7 was lymphocyte rich and depleted of progenitor cell activity. Transplantation of CP 1-7 led to an eventual lymphohemopoietic reconstitution of irradiated monkeys; however, complete engraftment was delayed by as much as 14 days compared to either the transplantation of CP 8-10 or to unfractionated bone marrow. Thus, a presence of the HSC in the lymphocyte-rich progenitor-cell depleted population can be detected in the rhesus model.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1986
Accession Number
ADA192769

Entities

People

  • Gretchen N. Schwartz
  • John H. Darden
  • Myra L. Patchen
  • Rod L. Monroy
  • Thomas J. Macvittie

Organizations

  • Armed Forces Radiobiology Research Institute

Tags

Communities of Interest

  • Advanced Electronics
  • Autonomy

DTIC Thesaurus Topics

  • Animals
  • Antigens
  • Blood
  • Blood Cells
  • Bone Marrow
  • Bone Marrow Cells
  • Cardiovascular System
  • Cells
  • Culture Media
  • Hematology
  • Lymphatic System
  • Lymphocytes
  • Myeloid Cells
  • Rhesus Monkeys
  • Stem Cells

Fields of Study

  • Biology
  • Medicine

Readers

  • Aquatic Ecology
  • Immunology
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech